Aklides is a multicomponent system:
- a high quality fluorescence microscope
- a LED lightsource (autonomy > 100000 hours)
- a motorized scanning stage
- a CCD camera
- Aklides® software

The Aklides® software is the heart of the system, providing the algorithms for optimal image generation (positioning, focusing, quality control), interpretation and pattern recognition. Fluorescence intensity is quantified in arbitrary units, allowing better standardization in autoimmunity testing. Images and results are saved in the system data bank and included into patient's reports at the pdf format. Results can be easily exchanged between laboratories.

Image acquisition in the standard mode takes less than 1 minute per well for HEP-2 testing, including pattern recognition. A screening mode for differentiation between positive and negative samples allows processing of 64 wells within a few minutes.

In its current version, Aklydes has 4 different modules:
- ANA (HEp-2): positive/negative interpretation and pattern recognition.
- nDNA (Crithidia lucilliae): positive/negative interpretation.
- ANCA: differenciation between c-ANCA and p-ANCA.

The software is user-friendly: the user chooses the slide format, key in or import patient data, loads the slides (up to 4 in the standard configuration) and starts the reading. The complete process takes between 15 minutes (nDNA) and 60 minutes (HEp-2) for 4 slides. Each result has to be validated (or modified) by the user before storage in the data bank. 

All pictures and results are automatically saved and can be extracted to generate several reports (per slide, per patient, per autoantibody, etc). Teaching hospitals will also greatly benefit from the system.

Aklides® software has been developped for reagents specially optimalized for the system (list on the right), although it can be used as a conventional microscope in open mode, but without automated slide processing. In this configuration, the user can take the pictures of his choice without the need of a dark room and store them in the system archives.

Presentation of the system 

Principle of the system

AKLIDES® REAGENTS

ANA (HEp-2) - Automated reading and pattern recognition:
- ANA (30x16) - ref. 4058
- ANA (40x12) - ref. 4068

nDNA (Crithidia) - Automated reading and evaluation:
- nDNA (10x10) - réf. 4078

ANCA (granulocytes) - Automated reading and evaluation:
- c-ANCA (10x6) - ref. 4060
- p-ANCA (10x6)

Others (reagents optimalized for Aklides in open mode):
- AMA (12x4) - ref. 83048
- AMA (12x8) - ref. 83096
- ASMA (12x4) - ref. 84048
- ASMA (12x8) - ref. 84096
- Triple (12x4) - ref. 85048
- Triple (12x8) - ref.85096
- Anti-GBM (12x4) - ref. 86448
- Anti-GBM (12x8) - ref. 86496
- ASA (12x4) - ref. 86148
- ASA (12x8) - ref. 86196
- ATA (12x4) - ref. 85548
- ATA (12x8) - ref. 85596
- EmA (12x4) - ref. 86048
- EmA (12x8) - ref. 86096
- CMA (12x4) - ref. 86248
- CMA (12x8) - ref. 86296
- SkMA (12x4) - ref. 86348
- SkMA (12x8) - ref. 86396


 LINKS

www.aklides.com

Labodia autoimmunity productlist

ATLAS ANA