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TPO Orkit®
Cat. No TPO-100: 96
well kit
| Introduction |
Thyroperoxidase (TPO)
has been found to be the main component of the “microsomal
antigen”. The presence of TPO autoantibodies in serum is
associated with thyroid autoimmune diseases (Graves’ disease and
Hashimoto’s thyroiditis). These antibodies predominantly belong
to the IgG class and are clearly polyclonal.TPO
antibody testing is especially helpful in case of:
- clinical suspicion of Graves’ disease without thyroid or
ocular signs,
- subclinical hypothyroidism (TSH between 5 and 20 mIU/l),
- suspicion of hypothyroidism in school children.
Elevated TPO
antibody levels in early pregnancy represent a risk factor for
post partum thyroiditis. High
TPO antibody concentrations should also be taken into account
before initiating a treatment with amiodarone, lithium or alpha
interferon.
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| Main
characteristics |
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Quantitative
determination.
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Result
expression in international units
(MRC 66/387)
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Ready-to-use
Calibrators with individual color-coding.
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Ready-to-use,
stable Conjugate and Chromogen for simpler handling
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Breakaway
wells, ideally suited for small runs and for maximum
cost-efficiency..
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Common
sample dilution protocol for thyroglobulin and
thyroperoxidase autoantibody testing allowing simultaneous
determination of both antibodies.
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Room temperature
incubations, standardized pipetting volumes
and protocol for easy handling or adaptation to ELISA
automatic processors
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Non-specific
reagents (Wash Buffer, Chromogen, Stop Solution) are
common to all Orkit assays and can be used across the
entire product line, regardless of batch numbers.
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| Practical
information |
Kit insert and other
documents
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| Samples
Human serum only (10 µl)
Kit contents
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Human
thyroid peroxidase coated microwells (96 wells) |
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IgG
Conjugate (11 ml)
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Calibrators
(1 ml each): 0, 30, 90, 300 and 1000 IU/ml
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Positive
Control
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Diluent,
Wash Buffer, TMB and Stop Solution
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Assay procedure
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TPO
IgG |
Dilute
samples |
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Add
100 µl samples / Calibrators / Control |
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Incubate 30 min. (Room
Temperature) |
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Wash |
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Add
Conjugate: 100 µl |
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Incubate 30 min. (Room
Temperature) |
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Wash |
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Add Substrate: 100 µl |
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Incubate 30 min. (Room
Temperature) |
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Add Stop Solution:
100 µl |
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Read at 450 nm |
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| Assay
performances |
Sensitivity
Minimum detectable dose: 2
IU/ml
Precision
| r=12 |
Sample
1 |
Sample
2 |
Sample
3 |
| Mean concentration (IU/ml) |
48 |
124 |
234 |
| Standard deviation (IU/ml) |
3.14 |
4.48 |
21.5 |
| Coefficient of variation (%) |
6.5 |
3.6 |
9.3 |
Reproducibility
| n=8 |
Sample
1 |
Sample
2 |
Sample
3 |
| Mean concentration (IU/ml) |
48 |
161 |
234 |
| Standard deviation (IU/ml) |
4.9 |
23.6 |
50.2 |
| Coefficient of variation (%) |
10 |
14 |
17 |
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| Expected
results |
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