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| Introduction: |
A lysosomal heme protein that catalyzes oxidations by hydrogen peroxide, including MPO–chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, cross–linking of proteins, and iodination of proteins.
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| Source: |
Prepared from blood that tested negative for HBsAg and for HIV and HCV antibodies. It is recommended that this product be handled using established good laboratory practices since no test method can guarantee that products derived from human blood will not transmit infection.
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| Form: |
Solid, lyophilized in 50 mM sodium acetate, pH 6.0, and 100 mM NaCl.
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| Amount per kit: |
100 µg
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| CAS: |
9003-99-0
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| EC: |
1.11.1.7
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| Weight: |
118,000
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| Purity: |
>=95% by SOS
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| Spec Act: |
50–200 units/mg protein
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| Unit: |
One unit is defined as the amount of enzyme that will hydrolyze 1.0 mmol of H2O2 per minute at 25 degrees C, pH 6.0.
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| Solubility: |
Reconstitute with 1 mL of 50 mM sodium acetate, pH 6.0, and 100 mM NaCl. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H2O.
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| Storage: |
Freeze. Following reconstitution, aliquot and refreeze at –20 degrees C or below. This product is stable for 2 years as supplied. Stock solutions are stable for 6–12 months at –20 degrees C or below.
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| References: |
1.Panasenko, O.M., et al. 1994. Free Radical Biol. Med. 16, 143.
2.Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
3.Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.
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**For research use only. Not for use in diagnostic procedures.** |