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| Introduction: |
Affinity purified, biologically active, full length recombinant GST P1–1. GSTs catalyze the formation of the thioester conjugates between glutathione and reactive xenobiotic compounds and are believed to be important for the cellular response to oxidative stress. Suitable for procarcinogen studies. Higher levels of GST P1–1 are reported in many human tumors, including melanomas. GST P1–1 is widely distributed throughout the body, except for the liver.
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| Form: |
Liquid. Supplied in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.
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| Amount per kit: |
100 µg.
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| Weight: |
50,000
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| Purity: |
> 95% by SDS-PAGE
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| Spec Act: |
75 units/mg protein
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| Unit: |
One unit is defined as the amount of enzyme that will conjugate 1.0 mmol of 1–chloro–2,4–dinitrobenzene (CDNB) to reduced glutathione per minute at 20 degrees C, pH 6.5.
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| Storage: |
Freeze. This product is stable for 1 year as supplied.
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| References: |
1.Kolm, R.H., et al. 1995. Biochem. J. 311, 453.
2.Stenberg, G., et al. 1992. Protein Expression Purif. 3, 80.
3.Widersten, M., et al.1991. Biochem. J. 276, 519.
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**For research use only. Not for use in diagnostic procedures.** |