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| Introduction: |
Long–acting, extremely stable form of catalase that is active from pH 2–12. Composed of four protein subunits, each containing a heme (Fe3+–protoporphyrin) group bound to its active site. Each subunit also contains one molecule of bound NADPH that helps to stabilize the enzyme. Catalyzes the reaction 2H2O2¨2H2O+O 2. Catalase, along with superoxide dismutase and glutathione peroxidase, controls the level of oxygen–derived free radicals in mammalian cells, and together may function as a somatic oxidant defense. Prevents apoptosis in cultured rat ovarian follicles.
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| Form: |
Light brown lyophilized solid
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| Amount per kit: |
100 KU
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| CAS: |
9001–05–2
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| RETCS: |
FI4378000
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| EC: |
1.11.1.6
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| Weight: |
250,000
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| Contaminates: |
Microbial contamination: >50 cfu/g dry weight
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| Spec Act: |
10 KU/mg protein
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| Activity: |
>4 KU/mg dry weight
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| Unit: |
1 KU = 1000 units. One unit is defined as the amount of enzyme that will decompose 1.0 µmol of H2O2 per minute at 30 degrees C, pH 7.0.
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| Solubility: |
H2O (20 mg/ml) and physiological buffers
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| Storage: |
Freeze. Following reconstitution, aliquot and refreeze for long term storage or store at room temperature for short term storage. This product is stable for 2 years as supplied. Aqueous stock solutions are stable for several weeks at room temperature or for several months frozen.
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| References: |
1.Tilly, J.L., and Tilly, K.I. 1995. Endocrinology 136, 242.
Kassim, E.A. 1982. Microbiol. Immunol. 26, 449.
2.Kikuchi
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**For research use only. Not for use in diagnostic procedures.** |